| 產(chǎn)品編號 | bsm-61358R |
| 英文名稱 | MGMT Recombinant Rabbit mAb |
| 中文名稱 | O6甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶重組兔單抗 |
| 別 名 | MGMT |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測分子量 | 22 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MGMT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. SWISS: P16455 Gene ID: 4255 O-6-methylguanine-DNA methyltransferase(MGMT)O-6-甲基鳥嘌呤-DNA-甲基轉(zhuǎn)移酶:是廣泛存在于細菌到哺乳動物機體中的一種獨特DNA修復(fù)酶，MGMT是一種DNA修復(fù)酶，是突變和癌變的抑制因子。它對烷化劑導(dǎo)致的DNA損傷有修復(fù)作用。 MGMT是突變和癌基因的抑制因子。研究表明MGMT水平與惡性腫瘤的發(fā)生、進展、浸潤、預(yù)后及治療有密切關(guān)系。細胞中MGMT水平與其所能耐受的DNA損傷密切相關(guān)。一般來講，低水平表達MGMT的腫瘤可以應(yīng)用烷化劑藥物治療；反之則為耐藥。在 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with MGMT monoclonal antibody, unconjugated (bsm-61358R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MGMT Monoclonal Antibody, Unconjugated(bsm-61358R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MGMT Monoclonal Antibody, Unconjugated(bsm-61358R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
