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Dnmt1 Recombinant Rabbit mAb (bsm-61399R)  
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25ul/800.00元
50ul/1400.00元
100ul/2500.00元
大包裝/詢價(jià)

產(chǎn)品編號(hào) bsm-61399R
英文名稱 Dnmt1 Recombinant Rabbit mAb
中文名稱 DNA甲基轉(zhuǎn)移酶1重組兔單抗
別    名 ADCADN; CXXC finger protein 9; CXXC9; DNA methyltransferase 1; DNA MTase; Dnmt1o; HSN1E; M.HsaI; MCMT; Met1; MommeD2.  
抗體來源 Rabbit
克隆類型 Recombinant
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 183 kDa
檢測(cè)分子量 183
性    狀 Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human DNMT1 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
保存條件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2.

SWISS:
P26358

Gene ID:
1786

DNA甲基轉(zhuǎn)移酶-1在多種腫瘤細(xì)胞中表達(dá)量相當(dāng)高,而在正常成人細(xì)胞中則低表達(dá),因此dnmt1基因的高表達(dá)與腫瘤的發(fā)生有密切的關(guān)系。 dnmt-1 對(duì)細(xì)胞周期、增殖及凋亡有一定的的影響。細(xì)胞核表達(dá)。 (isoform CRA_a)為抑制細(xì)胞增殖、促進(jìn)細(xì)胞凋亡,為腫瘤的基因治療提供依據(jù)。
產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with Dnmt1 monoclonal antibody, unconjugated (bsm-61399R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated(bsm-61399R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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