產(chǎn)品編號 | bsm-61405R |
英文名稱 | Parvalbumin Recombinant Rabbit mAb |
中文名稱 | 細小清蛋白重組兔單抗 |
別 名 | Parvalbumin alpha; PVALB. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | 11H20 |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:100-1:500,IHC-F=1:100-1:500,IF=1:100-1:500,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 12 kDa |
檢測分子量 | 12 |
性 狀 | Liquid |
免 疫 原 | KLH conjugated synthetic peptide derived from human Parvalbumin alpha |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. SWISS: P20472 Gene ID: 5816 (Parvalbumin, PV)細小白蛋白屬于鈣結(jié)合蛋白家族,是一種細胞內(nèi)可溶的小結(jié)合蛋白,過去曾認為,僅出現(xiàn)在哺乳動物某些肌纖維內(nèi),它與Ca2+有高度親合力,與鈣調(diào)蛋白競爭自由Ca2+,故可阻止肌漿內(nèi)的Ca2+增高,可抗衡細胞內(nèi)鈣過量的發(fā)生。 |
產(chǎn)品圖片 |
Western blot analysis of human cerebellum lysate. Using Parvalbumin (bsm-61405R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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