| 產(chǎn)品編號 | bsm-61441R |
| 英文名稱 | Cathepsin B Recombinant Rabbit mAb |
| 中文名稱 | 組織蛋白酶B重組兔單抗 |
| 別 名 | APPS; Cathepsin B heavy chain; Cathepsin B1; CathepsinB; CPSB; CTSB; cysteine protease; Preprocathepsin B. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 14C12 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | 38 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Cathepsin B: 2-180 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. SWISS: P07858 Gene ID: 1508 合成與降解（Synthesis and Degradation） 組織蛋白酶B(CB) 參與腫瘤的侵襲轉(zhuǎn)移過程。可促進腫瘤血管的增生，增強腫瘤細胞的運動能力.組織蛋白酶B轉(zhuǎn)錄以及表達水平的高低與腫瘤預后呈正相關。 cathepsin B屬于半胱氨酸蛋白酶,與人體的多種生理、病理過程密切相關.組織蛋白酶B (cathepsin B,CB)主要存在于溶酶體中, 發(fā)揮其生理狀態(tài)下的蛋白水解作用.但在某些特定條件下, CB在胞外間質(zhì)中有時也可以檢測到. 目前，有學者將CB用于阿爾茨海默?。ˋ |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Cathepsin B monoclonal antibody, unconjugated (bsm-61441R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cathepsin B Monoclonal Antibody, Unconjugated(bsm-61441R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cathepsin B Monoclonal Antibody, Unconjugated(bsm-61441R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cathepsin B Monoclonal Antibody, Unconjugated(bsm-61441R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cathepsin B Monoclonal Antibody, Unconjugated(bsm-61441R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cathepsin B Monoclonal Antibody, Unconjugated(bsm-61441R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cathepsin B Monoclonal Antibody, Unconjugated(bsm-61441R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
