| 產(chǎn)品編號(hào) | bs-1147R |
| 英文名稱 | TSLC1 Rabbit pAb |
| 中文名稱 | 細(xì)胞粘附分子1抗體 |
| 別 名 | CADM1; BL2; Cadm1; CADM1_HUMAN; Cell adhesion molecule 1; DKFZp686F1789; IGSF4; IGSF4A; Immunoglobulin Superfamily Member 4; MGC149785; MGC51880; NECL-2; NECL2; Nectin like 2; Nectin-like protein 2; RA175; SgIGSF; Spermatogenic immunoglobulin superfamily; ST17; sTSLC 1; Synaptic cell Adhesion Molecule; SynCAM; SynCAM1; TSLC-1; TSLC1; Tumor Suppressor in Lung Cancer 1. |
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Specific References (3) | bs-1147R has been referenced in 3 publications.
[IF=4.2] Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829. WB ; Mouse.
[IF=3.98] Liu, Wen-bin, et al. "Aberrant methylation accounts for cell adhesion-related gene silencing during 3-methylcholanthrene and diethylnitrosamine induced multistep rat lung carcinogenesis associated with overexpression of DNA methyltransferases 1 and 3a." Toxicology and applied pharmacology 251.1 (2011): 70-78. IHC-P ; Rat.
[IF=3.923] Pan T et al. MiR-128-1-5p regulates tight junction induced by selenium deficiency via targeting cell adhesion molecule 1 in broilers vein endothelial cells.J Cell Physiol. 2018 Nov;233(11):8802-8814. WB ; broiler.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TSLC1: 365-442/442 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. Function: Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. Subunit: Homodimer. Interacts with CRTAM and EPB41L3/DAL1. The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton. Interacts via its C-terminus with the PDZ domain of MPP3 and the PDZ domain of MPP6. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder. Similarity: Belongs to the nectin family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9BY67 Gene ID: 23705 Database links: Entrez Gene: 23705 Human Entrez Gene: 54725 Mouse Omim: 605686 Human SwissProt: Q9BY67 Human SwissProt: Q8R5M8 Mouse Unigene: 370510 Human Unigene: 234832 Mouse TSLC1屬于新的抑癌基因免疫球蛋白超家族, TSLC1蛋白質(zhì)參與細(xì)胞間黏附、細(xì)胞運(yùn)動(dòng)、信號(hào)轉(zhuǎn)導(dǎo)及免疫調(diào)節(jié). TSLC1在多種腫瘤中表達(dá)異常,它對(duì)腫瘤的影響主要表現(xiàn)為抑制瘤細(xì)胞增殖及誘導(dǎo)凋亡、改變瘤細(xì)胞的生長(zhǎng)特性及基因表達(dá),可抑制上皮間質(zhì)轉(zhuǎn)化. TSLC1蛋白在控制腫瘤生物侵襲性方面有著重要作用，也可能作為預(yù)測(cè)患者預(yù)后的重要生物標(biāo)記，但是TSLC1的腫瘤抑制分子機(jī)制還有待進(jìn)一步研究。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: U2os (Human) Cell Lysate at 30 ug
Lane 4: 293T (Human) Cell Lysate at 30 ug
Primary: Anti-TSLC1 (bs-1147R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 100 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SynCAM/TSLC1 Polyclonal Antibody, Unconjugated(bs-1147R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SynCAM/TSLC1 Polyclonal Antibody, Unconjugated(bs-1147R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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