| 產品編號 | bs-3524R |
| 英文名稱 | PEA15 Rabbit pAb |
| 中文名稱 | 星形膠質細胞PEA15抗體 |
| 別 名 | Astrocytic phosphoprotein PEA 15; Astrocytic phosphoprotein PEA15; HMAT 1; HMAT1; Homolog of mouse MAT 1 oncogene; Homolog of mouse MAT1 oncogene; HUMMAT 1H; HUMMAT1H; MAT 1; MAT 1H; MAT1; MAT1H; PEA 15; PEA-15; PEA15 protein; PED; phosphoprotein enriched |
| 研究領域 | 免疫學 神經生物學 生長因子和激素 轉錄調節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PEA15: 51-130/130 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
PED/PEA 15 (Phosphoprotein Enriched in Diabetes/Phosphoprotein Enriched in Astrocytes 15 kDa) is a widely expressed 15 kDa protein comprised of an N terminal region containing a canonical Death Effector Domain (DED) sequence and a nuclear export signal, and a C terminal region containing two serine phosphorylation sites. PED/PEA 15 has been implicated in the regulation of multiple cellular processes including apoptosis, integrin activation, and insulin sensitive glucose transport in insulin responsive cells. Phosphorylation of both serine 104 (a Protein Kinase C site) and serine 116 (a substrate of CaMKII and Akt) is required for PED/PEA 15 function. Function: Blocks Ras-mediated inhibition of integrin activationand modulates the ERK MAP kinase cascade. Inhibits RPS6KA3activities by retaining it in the cytoplasm (By similarity).Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity andapoptosis. Regulates glucose transport by controlling both thecontent of SLC2A1 glucose transporters on the plasma membrane andthe insulin-dependent trafficking of SLC2A4 from the cell interiorto the surface. Subunit: Binds RPS6KA3, MAPK3 and MAPK1. Transient interactionwith PLD1 and PLD2 (By similarity). Interacts with CASP8 and FADD. Subcellular Location: Cytoplasm. Note=Associated withmicrotubules. Tissue Specificity: Ubiquitously expressed. Most abundant intissues such as heart, brain, muscle and adipose tissue whichutilize glucose as an energy source. Lower expression inglucose-producing tissues. Higher levels of expression are found intissues from individuals with type 2 diabetes than in controls. Post-translational modifications: Phosphorylated by protein kinase C andcalcium-calmodulin-dependent protein kinase. These phosphorylationevents are modulated by neurotransmitters or hormones. Similarity: Contains 1 DED (death effector) domain. SWISS: Q15121 Gene ID: 8682 Database links: Entrez Gene: 8682 Human Entrez Gene: 18611 Mouse Omim: 603434 Human SwissProt: Q15121 Human SwissProt: Q62048 Mouse Unigene: 517216 Human Unigene: 544 Mouse |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
