| 產(chǎn)品編號 | bs-6335R |
| 英文名稱 | ALOX15B Rabbit pAb |
| 中文名稱 | 花生四烯酸15脂氧合酶2抗體 |
| 別 名 | 15 LO 2; 15 LOX 2; 15-lipoxygenase 2; 15-LOX-2; 15-LOX-B; ALOX15B; Arachidonate 15 lipoxygenase second type; Arachidonate 15 lipoxygenase type II; Arachidonate 15-lipoxygenase B; Arachidonate 15-lipoxygenase type II; LX15B_HUMAN; ALOX8_MOUSE; Arachidonate |
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Specific References (1) | bs-6335R has been referenced in 1 publications.
[IF=5.502] Xurui Li. et al. p53 activates the lipoxygenase activity of ALOX15B via inhibiting SLC7A11 to induce ferroptosis in bladder cancer cells. LAB INVEST. 2023 Jan;:100058 IHC ; Human.
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| 研究領(lǐng)域 | 腫瘤 心血管 細胞生物 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALOX15B/15 Lipoxygenase 2: 13-110/676 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Tissue specificity: Expressed in hair, prostate, lung and cornea. Function: Converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in hair, prostate, lung and cornea. Similarity: Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain. SWISS: O15296 Gene ID: 247 Database links: Entrez Gene: 247 Human Entrez Gene: 11688 Mouse Omim: 603697 Human SwissProt: O15296 Human SwissProt: O35936 Mouse SwissProt: Q5NCF6 Mouse SwissProt: Q5NCF7 Mouse Unigene: 111256 Human Unigene: 289672 Mouse Unigene: 86437 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALOX15B ) Polyclonal Antibody, Unconjugated (bs-6335R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALOX15B ) Polyclonal Antibody, Unconjugated (bs-6335R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALOX15B) Polyclonal Antibody, Unconjugated (bs-6335R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
