| 產(chǎn)品編號 | bs-10958R |
| 英文名稱 | SLC32A 1 Rabbit pAb |
| 中文名稱 | 氨基丁酸轉運蛋白VGAT抗體 |
| 別 名 | VGAT; bA122O1.1; GABA and glycine transporter; hVIAAT; SLC32A 1; solute carrier family 32(GABA vesicular transporter) member 1; Solute carrier family 32 member 1; Vesicular GABA Amino Acid Transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT; VIAAT_HUMAN. |
| 研究領域 | 細胞生物 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC32A1: 1-100/525 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Synaptic transmission involves the controlled exocytosis of vesicles containing specific neurotransmitters. Usually, neurotransmitters are synthesized in the cytoplasm of the cell and must be transported into synaptic vesicles for release. The vesicular GABA transporter (VGAT) is responsible for loading gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter, from neuronal cytoplasm into synaptic vesicles and is expressed only in the nerve endings of inhibitory neurons that contain GABA and/or glycine. During neocortical development, VGAT expression barely precedes the maturation of inhibitory synaptogenesis, suggesting that it may contribute to the development of neocortical GABAergic circuitry. VGAT may also play a role in epileptogenesis and the recovery mechanisms that occur after a spontaneous seizure Function: Involved in the uptake of GABA and glycine into the synaptic vesicles. Subcellular Location: Cytoplasmic vesicle membrane; Multi-pass membrane protein. Tissue Specificity: Retina. Expressed throughout the horizontal cells or more specifically at the terminals. Similarity: Belongs to the amino acid/polyamine transporter 2 family. SWISS: Q9H598 Gene ID: 140679 Database links: Entrez Gene: 140679 Human Entrez Gene: 22348 Mouse SwissProt: Q9H598 Human SwissProt: O35633 Mouse Unigene: 179080 Human Unigene: 143404 Mouse Unigene: 413854 Mouse Unigene: 10846 Rat |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with SLC32A 1 polyclonal antibody, unconjugated (bs-10958R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
