| 產(chǎn)品編號(hào) | bsm-60578R |
| 英文名稱 | SUV39H2 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | D8C12 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 使用單甲基化 H3“Lys-9”作為底物，特異性三甲基化組蛋白 H3 的“Lys-9”的組蛋白甲基轉(zhuǎn)移酶。H3 'Lys-9' 三甲基化代表通過將 HP1（CBX1、CBX3 和/或 CBX5）蛋白募集到甲基化組蛋白來抑制表觀遺傳轉(zhuǎn)錄的特定標(biāo)簽。主要在異染色質(zhì)區(qū)域起作用，從而在中心和端粒區(qū)域的組成型異染色質(zhì)的建立中發(fā)揮核心作用。 H3 'Lys-9' 三甲基化也需要指導(dǎo) DNA 甲基化在中心重復(fù)。SUV39H1 通過與 RB1 的相互作用靶向組蛋白 H3，并參與許多過程，如細(xì)胞周期調(diào)節(jié)、轉(zhuǎn)錄抑制和端粒長度調(diào)節(jié)?？赡軈⑴c精子發(fā)生過程中高級(jí)染色質(zhì)組織的調(diào)節(jié)。由大 PER 復(fù)合物招募到晝夜節(jié)律靶基因的 E-box 元件，如 PER2 本身或 PER1，有助于通過 H3 'Lys-9' 三甲基化將局部染色質(zhì)轉(zhuǎn)化為異染色質(zhì)樣抑制狀態(tài)。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: SHSY-5Y cell lysate, the SUV39H2 Rabbit mAb pre-adsorbed with 3μM of the synthetic peptides;
2: SHSY-5Y cell lysate, the SUV39H2 Rabbit mAb with no peptide blocking.
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 47 kDa
Observed MW: 65 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: SHSY-5Y, 2: MOLT-4, 3: NIH-3T3, 4: C6, 5: Mouse testis, 6: Rat testis
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 47 kDa
Observed MW: 65 kDa
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