| 產(chǎn)品編號 | bsm-60369R |
| 英文名稱 | SCGN Recombinant Rabbit mAb |
| 中文名稱 | 促泌素重組兔單抗 |
| 別 名 | SEGN_HUMAN; Secretagogin; SECRET; secretagogin, EF-hand calcium binding protein; SEGN; CALBL; setagin; DJ501N12.8; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | H3A1 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | IHC-P=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008] Subcellular Location: Cytoplasm. Secreted.Cytoplasmic vesicle, secretory vesicle membrane; Peripheralmembrane protein; Cytoplasmic side.Note=Predominantly cytoplasmic. A small proportion is associatedwith secretory granules and membrane fractions.Detectable in human serum after ischemic neuronal damage. Tissue Specificity: Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons. Database links: Entrez Gene: 10590 Human SwissProt: O76038 Human 該蛋白是在細胞質(zhì)中發(fā)現(xiàn)的一種分泌的鈣結(jié)合蛋白。它與鈣調(diào)蛋白D-28K和鈣調(diào)蛋白有關(guān)。該蛋白被認為與KCL刺激的鈣通量和細胞增殖有關(guān)。與SCGN相關(guān)的疾病包括胰島素瘤和神經(jīng)內(nèi)分泌腫瘤。其相關(guān)途徑包括Neuroscience和Ca，cAMP和脂質(zhì)信號傳導。該基因的重要旁系同源物是CALB2。 |
| 產(chǎn)品圖片 |
Tissue: Rat pancreas
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60369R
Tissue: Mouse pancreas
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60369R
Tissue: Human pancreas
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60369R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
