| 產(chǎn)品編號 | bsm-60574R |
| 英文名稱 | ELP3 Recombinant Rabbit mAb |
| 中文名稱 | 組蛋白乙酰轉(zhuǎn)移酶KAT9重組兔單抗 |
| 別 名 | ELP3_HUMAN; Elongator complex protein 3; EC:2.3.1.-; hELP3; tRNA uridine(34) acetyltransferase; elongator acetyltransferase complex subunit 3; KAT9; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | E9G9 |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000,IHC-P=1:50-1:100,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ELP3 is the catalytic subunit of the histone acetyltransferase elongator complex, which contributes to transcript elongation and also regulates the maturation of projection neurons (Creppe et al., 2009 [PubMed 19185337]).[supplied by OMIM, Apr 2009] Database links: Entrez Gene: 55140 Human Entrez Gene: 74195 Mouse SwissProt: Q9H9T3 Human SwissProt: Q9CZX0 Mouse RNA聚合酶II延伸復(fù)合體的催化tRNA乙酰轉(zhuǎn)移酶亞基，是RNA聚合酶II（Pol II）全酶的組成部分，參與轉(zhuǎn)錄伸長。多個tRNA修飾需要拉伸配合物，包括mcm5U（5-甲氧基羰基甲基尿苷）、mcm5s2U（5-甲氧基羰基甲基-2-硫脲定）和ncm5U（5-氨基甲酰甲基尿苷）。在拉長復(fù)合物中，通過調(diào)節(jié)tRNAs中34位的擺動堿基中羧甲基尿苷的形成，充當(dāng)tRNA尿苷（34）乙酰轉(zhuǎn)移酶。也可以通過介導(dǎo)目標(biāo)蛋白質(zhì)的乙?；鳛榈鞍踪|(zhì)賴氨酸乙酰轉(zhuǎn)移酶。然而，這種活性在體內(nèi)尚不清楚，最近的證據(jù)表明ELP3主要作為tRNA乙酰轉(zhuǎn)移酶。還能參與神經(jīng)發(fā)生，通過依賴于α-微管蛋白乙?；倪^程調(diào)節(jié)投射神經(jīng)元在大腦皮層中的遷移和分支，也是發(fā)育中的大腦皮層中GJA1乙?；匦璧?。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, K562, Mouse brain, Raw264.7
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 62 kDa
Observed MW: 62 kDa
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60574R
Tissue: Mouse testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab:SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60574R
Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60574R
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