| 產(chǎn)品編號 | bsm-60617R |
| 英文名稱 | Ly6A Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | E12A2 |
| 交叉反應(yīng) | Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 干細(xì)胞抗原 1 (Ly6A) 是淋巴細(xì)胞抗原 6 (Ly-6) 家族的成員，是小鼠造血祖細(xì)胞/干細(xì)胞亞群的主要表型標(biāo)志物。Ly-6家族參與B細(xì)胞和T細(xì)胞活化的調(diào)節(jié)。Ly6A在多能造血干細(xì)胞 (HSC) 上表達(dá)，并用作小鼠 HSC 的標(biāo)志物。Ly6A在胎兒肝臟、成人骨髓、成人外周血和脾臟中普遍存在。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: C2C12 cell lysate, the Ly6A Rabbit mAb pre-adsorbed with 3μM of the synthetic peptides; 2: C2C12 cell lysate, the Ly6A Rabbit mAb with no peptide blocking.
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 14 kDa
Observed MW: 13 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: Mouse thymus, 2: C2C12
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 13 kDa
Tissue: Mouse testis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60617R
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