產(chǎn)品編號(hào) | bsm-60644R |
英文名稱(chēng) | Histone H2B (Crotonyl-Lys15 / Lys16 / Lys20) Recombinant Rabbit mAb |
中文名稱(chēng) | 巴豆酰組蛋白H2B重組兔單抗 |
別 名 | Crotonyl-Histone H2B(Lys15/Lys16/Lys20); Crotonyl-Histone H2B(Lys15/Lys16/Lys20); H2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e; H2B.f; H2B.j; H2B.q; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFJ; H2BFO; H2BFQ; H2BFS; HIRIP2; HIST1H2BB; HIST1H2BD; HIST1H2BH; HIST |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Recombinant |
克 隆 號(hào) | 1D12 |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 14 kDa |
檢測(cè)分子量 | 14 |
細(xì)胞定位 | 細(xì)胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised acetylpeptide derived from human Histone H2B around the Crotonyl site of Lys15 / Lys16 / Lys20 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] Subcellular Location: Secreted. SWISS: P33778 Gene ID: 3018 Database links: Entrez Gene: 3018 Human Omim: 602803 Human SwissProt: P33778 Human
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產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: (-): HeLa, (+): HeLa+ sodium
butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14kDa
Observed MW: 14kDa
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: (-): MCF-7, (+): MCF-7+ sodium
butyrate (50mM, 24hr) + trichostain A (500ng/ml,
4 hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14kDa
Observed MW: 14kDa
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