| 產(chǎn)品編號(hào) | bsm-61077R |
| 英文名稱 | TOP2A Recombinant Rabbit mAb |
| 中文名稱 | DNA拓普西異構(gòu)酶Ⅱ抗體 |
| 別 名 | DNA topoisomeraseⅡalpha; TOPO II; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme; TOP2; TP2A; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA Topoisomerase2; TOP 2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alp |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 11D3 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 174 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TOPO II alpha |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010] Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Subunit: Homodimer. Interacts with COPS5. Subcellular Location: Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm. Post-translational modifications: Phosphorylation has no effect on catalytic activity. Similarity: Belongs to the type II topoisomerase family. SWISS: P11388 Gene ID: 7153 Database links: Entrez Gene: 7153 Human Entrez Gene: 21973 Mouse Omim: 126430 Human SwissProt: P11388 Human SwissProt: Q01320 Mouse Unigene: 156346 Human Unigene: 4237 Mouse Unigene: 90996 Rat 拓?fù)洚悩?gòu)酶II是一種能催化DNA 拓?fù)洚悩?gòu)體相互變換，調(diào)節(jié)DNA三維結(jié)構(gòu)的核酶。與染色質(zhì)或染色體的組裝與分組裝、基因組復(fù)制、DNA重組、基因轉(zhuǎn)錄及DNA損傷后修復(fù)等有密切的關(guān)系。許多人的惡性腫瘤都有拓?fù)洚悩?gòu)酶II的過表達(dá)，因此，拓?fù)洚悩?gòu)酶II是許多化療藥的靶標(biāo)。大部分以拓?fù)洚悩?gòu)酶II為重要靶標(biāo)抗腫瘤的化療藥物是通過穩(wěn)定的DNA與拓?fù)洚悩?gòu)酶II的復(fù)合物激發(fā)凋亡的。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with TOP2A monoclonal antibody, unconjugated (bsm-61077R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated(bsm-61077R) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated(bsm-61077R) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated(bsm-61077R) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated(bsm-61077R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated(bsm-61077R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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